ISSN:
1438-2199
Keywords:
Amino acids
;
Nitric oxide
;
Endothelial cells
;
Glutathione
;
Polyomavirus
;
N-(2-Mercaptopropionyl)-glycine
;
1-Chloro-2,4-dinitrobenzene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The synthesis of nitric oxide (NO), detected as citrulline production, in human (HUVEC) and murine (tEnd.1) endothelial cells correlated with intracellular GSH. tEnd.1, which exhibited an intracellular GSH level 2.5-fold higher than HUVEC, showed a citrulline production (basally and after ionomycin stimulation) 5–8 times higher than human cells. Ionomycinelicited citrulline synthesis in tEnd.1 cells increased 2.4-fold after loading with GSH, and decreased dose-dependently after GSH depletion. Cell loading with N-(2-mercaptopropionyl)-glycine neither significantly increased citrulline production nor relieved the effect of GSH depletion.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00807330
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