ISSN:
1432-2242
Keywords:
Anther culture
;
Embryoids
;
Potato
;
Diploid
;
Microspores
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Solatium tuberosum L. diploid strains with superior androgenetic capacity have been selected for from androgenetic progenies of unselected diploid material. The paper also demonstrates that the use of a liquid medium for culturing potato anthers, instead of the conventional solid agar plates, improves the yield of androgenetic embryoids. The new method, associated with two successive cycles of selection for superior androgenetic response, allows the induction and regeneration of microspore derived plants on a large scale. The best genotype (clone 21 in this paper) regenerates androgenetic plants with a frequency around 30 per each anther plated. Over 80% of the regenerated plants are diploid. It is suggested that the androgenetic embryoids mainly originate from unreduced microspores by a mechanism which maintains a heterozygous or a partly heterozygous genetic situation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00251187
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