ISSN:
1619-7089
Keywords:
Key words: Technetium-99m bicisate
;
Preparation method
;
Microwave heating
;
Radiochemical purity
;
Thin-layer chromatography
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract. The method currently recommended for the preparation of technetium-99m bicisate (99mTc-bicisate) requires a lengthy 30-min incubation at room temperature. The purpose of this study was to evaluate an alternative method to shorten the preparation time. 99mTc-bicisate was prepared with 3.7 GBq (100 mCi) 99mTc according to the manufacturer’s instructions, except for the final incubation step, which was replaced with the microwave heating procedure. A standard thin-layer chromatography (TLC) method (i.e., Baker-Flex silica gel IB-F TLC plate with ethyl acetate as mobile phase) was used for the determination of the radiochemical purity (RCP) of 99mTc-bicisate. Our evaluation with different microwave heating processes (300 W with different heating times) demonstrated that as the microwave heating temperature was increased (i.e., 44º–71º C), an increased percentage of samples reached 95% within 5 min post preparation (n=58). The highest RCP value (i.e., 97.4%±0.5%, n=10) could be obtained immediately after an 8-s microwave heating time at 300 W (microwave temperature at 69º C), and an average RCP value of 96.4%±1.3% (n=90) was maintained throughout the 24-h evaluation period. However, the trend seemed to reverse at higher microwave temperatures (i.e., 76º–90º C), which reconfirmed our initial findings that overheating had no benefit for the preparation of 99mTc-bicisate. To ensure that temperature was the only determining factor, a hot water incubator set at 69º C was used (n=6). Similar RCP results were achieved. In conclusion, the use of a microwave oven at a low heat cycle provides a rapid and efficient way to prepare 99mTc-bicisate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00841404
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