ISSN:
1432-041X
Keywords:
Protein synthesis
;
Development
;
Antherea pernyi
;
Follicles
;
Specific ribonuclease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Crude, cell-free protein-synthesizing systems were prepared from follicles of two different stages of development in the ovariole of the silkmothAntherea pernyi. The efficiency of the translation of natural and synthetic mRNAs in these systems was compared with that in a cell-free wheat germ system. A postmitochondrial extract (S-30) from the follicles almost completely inhibited protein synthesis in a polyribosome-dependent, cell-free systems. A specific ribonuclease, obtained from the post mitochondrial extract by ammonium sulphate precipitation, heat denaturation and DEAE-cellulose chromatography, inhibited polyribosome-dependent protein synthesis. The effect of this specific ribonuclease on the structural integrity of radioactive RNAs and ribosomal subunits, which were isolated from Ehrlich ascites tumor cells, was also studied.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00848950
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