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  • Proteolysis  (1)
  • spatial variation  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The complete primary structure of mouse 20S proteasomes (1999)
    Springer
    Immunogenetics 49 (1999), S. 835-842 
    Details
    ISSN: 1432-1211
    Keywords: Key words Proteasome ; Antigen processing ; Multicatalytic proteinase ; Proteolysis ; Protein degradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The proteasome is a large multicatalytic proteinase that plays a role in the generation of peptides for presentation by major histocompatibility complex class I molecules. The 20S proteolytic core of mammalian proteasomes is assembled from a group of 17 protein subunits that generate a distinctive pattern of spots upon two-dimensional gel electrophoresis. The genes for most of these subunits have been cloned from humans and rats. We isolated cDNA clones for the mouse orthologues of ten of the subunits [PSMA1 (C2), PSMA2 (C3), PSMA3 (C8), PSMA4 (C9), PSMA5 (ZETA), PSMA6 (IOTA), PSMA7 (C6-I), PSMB2 (C7-I), PSMB3 (C10-II), and PSMB5 (X)] to complete the cloning of all of the mouse subunits. Using antisera raised against these subunits or their orthologues, we verified the identity of these proteins by two-dimensional NEPHGE-PAGE.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050562
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Spatial variability in secondary metabolite production by the tropical red alga Portieria hornemannii (1999)
    Springer
    Hydrobiologia 398-399 (1999), S. 263-273 
    Details
    ISSN: 1573-5117
    Keywords: monoterpene ; chemical variation ; spatial variation ; transplant ; secondary metabolites ; red algae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Apakaochtodenes A and B, which are halogenated monoterpenes and the major secondary metabolites in Portieria hornemannii, are effective feeding deterrents toward herbivorous reef fishes on Guam. A reciprocal transplant study was conducted to determine the relative importance of environmental versus genetic factors influencing site-to-site differences in the amount of apakaochtodenes produced. The study sites were chosen for characteristically high (Anae Island) and low (Gun Beach) apakaochtodene levels. Algae collected from Anae Island and Gun Beach differed significantly in concentrations of apakaochtodene B at the start of the experiment, but by the end they had almost the same amount of the metabolite because the level had decreased in plants at Anae Island. Additionally, algae from Anae Island had relatively high levels of apakaochtodene A (60–90% of apakaochtodene B concentration), whereas this compound was rarely detected in Gun Beach algae. Transplantation t o a different site had no significant effect on the levels of the apakaochtodenes, other than a decrease in concentration that might have resulted from handling the algae. Our data indicate a strong site-to-site difference in apakaochtodene levels in P. hornemannii on Guam, notable interplant variation in the levels of the compounds among thalli within the same site, and some evidence for temporal variation in levels of these compounds over a period of four weeks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1017003522096
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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