ISSN:
1432-2048
Keywords:
Daucus
;
Lectin
;
Nicotiana (protoplasts)
;
Protoplast (fusion, immobilization)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A technique is described for the reversible, lectin-mediated immobilization of plant protoplasts on agarose beads. Cyanogen-bromide-activated agarose beads were coated with protein (gelatine or bovine serum albumin) and lectins were subsequently linked to the protein layer using glutaraldehyde. The technique has possible applications in protoplast fusion-product isolation, cellrecognition studies, and membrane isolation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00982913
Permalink