ISSN:
1573-3297
Keywords:
Quantitative trait locus
;
recombinant inbred strains
;
open-field activity
;
mouse
;
genetics
;
Pomc1
;
proopiomelanocortin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Psychology
Notes:
Abstract Various lines of evidence suggest that a polymorphism in the gene for proopiomelanocortin, Pomc1, might account for some portion of the genetic variance for open-field activity in the LS × SS RI strains. To test this hypothesis, approximately 1600 bp of Pomc1was sequenced from genomic DNA of seven of the LS × SS strains. Two distinct alleles containing a total of five single-base pair differences were detected. A substitution was found in the coding region causing a Pro-to-Ser conversion, two substitutions occurred in the 3′ untranslated region of the mRNA, and a substitution and a deletion were detected in the 3′ untranscribed flanking region. The fragment containing the coding region substitution was sequenced in an additional 15 of the LS × SS strains. A total of 12 strains contained one form of the allele, while 10 had the other. The strain distribution pattern of open-field activity scores between the two alleles suggests that these alleles do not contribute to the genetic variation of open-field activity in the LS × SS RI strains.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1021408815540
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