ISSN:
1432-2242
Keywords:
Key wordsRAPD markers
;
Marker reliability
;
RAPD fragment sequence homology
;
Co-dominant bands
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The sequence homology of co-migrating RAPD markers within a genus, across species, and among populations of a species was investigated. DNA was isolated from ten wild Brassica species with and the RAPD patterns were established using three random primers. Five RAPD markers which appeared to be characteristic for the species (genus level), four markers which appeared to be species specific, and one population-specific marker were isolated from agarose gels and hybridized to the RAPD profiles of the ten Brassica species. Two RAPD markers were cloned for comparison with gel-isolated RAPD fragment probes in hybridization experiments. Non-specific and background hybridization, occurring when gel-isolated fragments were used as probes, disappeared when cloned fragments were used. A total of 250 RAPD-marker hybridizations were scored according to visual presence or absence in a gel lane. All except three markers hybridized as expected, resulting in an error rate of 1.2%. The deviating results included a lack of hybridization although a band was visible in the gel, a length polymorphism for one marker, and a dual hybridization signal for two single-band markers.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050240
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