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  • Rdy cat  (1)
  • dog chromosomes  (1)
  • flow karyotype  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Changes in a photoreceptor polypeptide correlating with an early-onset retinal dystrophy in the cat (1991)
    Springer
    Molecular and cellular biochemistry 107 (1991), S. 111-117 
    Details
    ISSN: 1573-4919
    Keywords: Rdy cat ; photoreceptor degeneration ; two-dimensional gel electrophoresis ; polypeptides ; rod outer segments ; visual transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract A preparation of rod outer segments has been used to study the polypeptides characteristic of an early-onset retinal dystrophy in cats (Rdy) by two-dimensional gel electrophoresis. Comparison of 2-D gels of rod outer segment preparations from retinas of normal and Rdy animals shows several differences. In particular, a polypeptide of Mr 51 kDa and pI 7.5 is present at increased levels in preparations from Rdy cats at 6 weeks, 9 weeks and 12.5 weeks of age but not at 3.5 weeks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225514
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Chromosome-specific paints from a high-resolution flow karyotype of the dog (1996)
    Springer
    Chromosome research 4 (1996), S. 115-123 
    Details
    ISSN: 1573-6849
    Keywords: chromosome painting ; dog chromosomes ; flow karyotype ; flow sorting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using peripheral blood lymphocyte cultures and duallaser flow cytometry, we have routinely obtained high-resolution bivariate flow karyotypes of the dog in which 32 peaks are resolved. To allow the identification of the chromosome types in each peak, chromosomes were flow sorted, amplified and labelled by polymerase chain reaction with partially degenerate primers and hybridized onto metaphase spreads of a male dog. The chromosome paints from 22 of the 32 peaks each hybridized to single homologue pairs and eight peaks each hybridized to two pairs. Paints from the remaining two peaks hybridized to only one homologue each in the male metaphase spread, thus corresponding to the sex chromosomes X and Y. All of the 38 pairs of autosomes and the two sex chromosomes of the dog could be accounted for in these painting experiments. The positions of chromosomes 1–21 were assigned to the flow karyotype (only chromosomes 1–21 have as yet been officially designated). The high-resolution flow karyotype and the chromosome paints will facilitate further standardization of the dog karyotype. The ability to sort sufficient quantities of dog chromosomes for the production of chromosome-specific DNA libraries has the potential to accelerate the physical and genetic mapping of the dog genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02259704
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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