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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Research in experimental medicine 170 (1977), S. 71-82 
    ISSN: 1433-8580
    Keywords: Renal transplantation ; inbred strains of rats ; alloimmune rosettes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 33 renal transplantations in the isohistogeneic rat strains DA/Han and BDE/Han were performed. The transplanted animals were killed at different days after transplantation and the following dates investigated: 1. number of rosette forming spleen cells with donor erythrocytes, calculated for 10.000 spleen cells and to the total number of extracted spleen cells, 2. haemagglutinin titers with donor erythrocytes, 3. titers of serum IgG, 4. total number of spleen cells, weight of spleens, weight and volumes of kidney transplants and the number of peripheral blood lymphocytes and granulocytes. The results demonstrate that the method of immunocytoadherence as described by BIOZZI and ZAALBERG in xenogeneic transplantation systems can also be used in allogenic systems. The course of the immune response was biphasic. Quantitation of allorosette forming cells can be considered as a parameter of allograft rejection. The current literature as to the T- or B-cell specificity of rosetting cells is discussed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Clinical and experimental medicine 147 (1968), S. 29-37 
    ISSN: 1591-9528
    Keywords: Renal transplantation ; Microsurgical technic ; Experiments on rats ; (Interesting for) Immunological experiments ; Nierentransplantation ; Mikrochirurgische Technik ; Rattenversuche ; (Interessant für) Immunologische Problemstellungen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird eine Technik zur Nierentransplantation bei Ratten vom Stamm Wistar/Han angegeben. Beim Spendertier wird ein Nieren-GefäßUreter-Präparat gewonnen; dabei dient ein Abschnitt der Vena cava zur Herstellung des Venenstreifens, der in die V. cava des Empfängertieres eingenäht wird. Die Aorta wird cranial zum Nierenarterienabgang unterbunden. Der caudale Abschnitt dient zu einer End-zu-Seit-Anastomose mit der Aorta des Empfängers. Dies bedeutet unseres Erachtens einen gewissen Vorteil gegenüber der Methode vonLee, weil die Anastomosenstellen weiter auseinander liegen und das Operationsgebiet übersichtlicher wird. Der Ureter mit anhängendem Harnblasenstück wird in Form einer Zipfelplastik in den oberen Pol der Empfängerblase eingenäht. Zur Zeit überleben rund 40% der Tiere die Operation. Der harmonische Mittelwert der überlebensdauer der Tiere beträgt z. Z. 26,4 Tage. Die Möglichkeit für die Bearbeitung zahlreicher Problemstellungen ist damit gegeben.
    Notes: Summary An improved technic for renal transplantation in rats of the strain Wistar/Han is described. The kidney is removed from the donor with parts of the vessels, the ureter and the bladder. A part of the V. cava is used as a patch and sutured with the vena cava of the host. The aorta is closed cranial to the renal artery, the caudal part is sutured as end-to-side-anastomosis to the aorta of the host. This is an advantage to the method ofLee because the two anastomoses are more distant one to each other and the technic becomes easier. The bladder of the donor animal is opened like an Y and sutured to the upper pole of the bladder of the host animal. 26.4 days is the harmonic mean value of the survival time and 40% of the rats survive renal transplantation. This gives possibilities to solve a number of experimental problems.
    Type of Medium: Electronic Resource
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