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  • 1
    Electronic Resource
    Electronic Resource
    Clinical utility of the flow cytometric technique for shared rheumatoid epitope (1999)
    Springer
    Rheumatology international 19 (1999), S. 31-34 
    Details
    ISSN: 1437-160X
    Keywords: Key words Shared rheumatoid epitope ; Rheumatoid arthritis ; Disease outcome ; Health assessment questionnaire ; Flow cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The shared rheumatoid epitope (SRE) on the MHC class II antigen-presenting molecule constitutes a probable genetic risk factor for the occurrence of rheumatoid arthritis (RA) and may also determine disease severity. We have used a novel flow cytometric technique to determine the SRE in over 500 predominantly Caucasian patients attending a general rheumatology clinic. This technique has been validated against a polymerase chain reaction (PCR)/SSO molecular method. The SRE was observed in 90% of patients with Felty's syndrome (n=10) and 75% of patients with RA (n=178) as compared with 39% of patients with osteoarthritis or non-inflammatory rheumatic disorders (n=73). Thus, the SRE determined by this method has a sensitivity for RA of 0.75, a specificity of 0.62 and an estimated positive predictive value of 0.02. In our RA cohort, there was no correlation between the functional outcome (health assessment questionnaire score) and SRE status. In conclusion, the determination of the SRE status by a flow cytometric method was found to have only modest sensitivity and specificity for RA; furthermore, the SRE did not correlate with functional outcomes. The clinical utility of the SRE assay is yet to be defined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002960050096
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitative evaluation of the growth of established cell lines on the surface of collagen, collagen composite and reconstituted basement membrane (1992)
    Springer
    Urological research 20 (1992), S. 285-288 
    Details
    ISSN: 1434-0879
    Keywords: Collagen ; Cell culture ; Urothelium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary As a step in the development of a system for assessing growth of human urothelium and transitional cell carcinoma, the growth of two established cell lines on collagen-based membranes has been evaluated. HT1080 (metastatic human fibrosarcoma) and WI38 VA13 (virus-transformed human fibroblasts) were grown on substrates of collagen, collagen/hyaluronic acid or chondroitin sulphate and reconstituted basement membrane (Matrigel). Cell growth was quantified using a new fluorimetric assay utilizing carboxyfluorescein diacetate. There were differences in morphology between cells grown on collagen and those grown on polystyrene. There were, however, no differences in growth of the WI38 VA13 cells on collagen compared with polystyrene, but growth of the HT1080 cells was increased on membranes of collagen/2:5% hyaluronic acid and collagen/5% chondroitin sulphate, and decreased on Matrigel. Adequate growth on collagen substrates is dependent on cell line. The fluorimetric assay used was suitable for quantifying cell growth on such substrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300260
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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