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Digitale Medien

Molecular methods of analysing bacterial diversity: the case of Rhizobia (1992)

Oyaizu, Hiroshi ; Naruhashi, Naohiro ; Gamou, Takuma

Springer

Biodiversity and conservation 1 (1992), S. 237-249

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ISSN: 1572-9710

Schlagwort(e): 16S rRNA ; Rhizobium ; Bradyrhizobium ; bacterial diversity ; DNA-DNA homology

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The distribution of rhizobia, bacteria which nodulate the roots of leguminous plants, was surveyed for 91 species of the familyLeguminosae. A total of 117 strains of rhizobia were isolated, and 28 strains were obtained from culture collections. The sample total of 145 strains was discriminated by rapid ribosomal RNA (rRNA) sequencing. The partial sequences (157 bases from position (inEscherichia coli) 1220 to 1377 from 5′ end) of 16S rRNA revealed the presence of 16 groups in these rhizobia. Further, DNA-DNA homology studies suggested that the differences of the 16 groups were enough to justify establishing at least 16 species.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00693762

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