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  • 1
    Electronic Resource
    Electronic Resource
    The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 101-109 
    Details
    ISSN: 1617-4623
    Keywords: Promoters ; S1 mapping ; In vitro transcription ; Protein secretion ; Signal peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The DNA sequence of a 1.77 kb region of the Streptomyces coelicolor chromosome containing the coding and regulatory regions of the extracellular agarase (dagA) gene was determined. The sequence predicts a primary translation product of 309 amino acids and Mr 35132. Comparison of the N-terminal sequence determined for the mature extracellular protein with that of the primary translation product deduced from the DNA sequence predicts the presence of a 30 amino acid signal peptide. Analysis of the transcription of the dagA gene using high resolution S1 mapping, in vitro transcription, dinucleotide-primed in vitro transcription and in vivo promoter probing identified four promoters, initiating transcription approximately 32, 77, 125 and 220 nucleotides upstream of the coding sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329843
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Organisation of the ribosomal RNA genes in Streptomyces coelicolor A3(2) (1988)
    Springer
    Molecular genetics and genomics 211 (1988), S. 191-196 
    Details
    ISSN: 1617-4623
    Keywords: Streptomyces coelicolor ; Ribosomal RNA genes ; Streptomyces lividans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using Southern hybridisation of radiolabelled purified ribosomal RNAs to genomic DNA the ribosomal RNA genes of Streptomyces coelicolor A3(2) were shown to be present in six gene sets. Each gene set contains at least one copy of the 5 S, 16 S and 23 S sequences and in at least two cases these are arranged in the order 16 S-23S-5S. Three gene sets, rrnB, rrnD and rrnF, were isolated by screening a λ library of S. coelicolor A3(2) DNA. The restriction map of one of these, rrnD, was determined and the nucleotide sequences corresponding to the three rRNAs were localised by Southern hybridisation. The gene order in rrnD is 16S-23S-5S.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330593
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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