ISSN:
1617-4623
Keywords:
Escherichia coli
;
Ribosomal protein
;
Nucleotide sequence
;
Physical map
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The rplY gene of Escherichia coli K12 encoding ribosomal protein L25 was cloned from the ordered clone bank and located at coordinate 2,291 kb on the physical map of E. coli. Determination of the nucleotide sequence indicated that the coding region contains 285 nucleotide pairs including a translational initiator and terminator. The amino acid sequence of the protein deduced from the nucleotide sequence matched completely the sequence determined for ribosomal protein L25. The coding region was found to be preceded by a typical promoter-like sequence and was followed by a DNA region capable of forming a secondary structure characteristic of a transcriptional terminator. Thus, the gene was concluded to constitute a transcriptional unit (operon). A preliminary analysis by Northern blot supported this conclusion. The codnn usage pattern of the rplY gene is characteristic of the ribosomal protein genes in E. coli.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00273625
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