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  • 1
    Electronic Resource
    Electronic Resource
    The secY-rpmJ region of the spc ribosomal protein operon in Escherichia coli: Structural alterations affecting secY expression (1989)
    Springer
    Molecular genetics and genomics 217 (1989), S. 1-5 
    Details
    ISSN: 1617-4623
    Keywords: Ribosomal protein ; secY ; Protein secretion ; Gene disruption ; rpmJ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A unique feature of the spc ribosomal protein operon is that its region distal to the promoter contains a gene (secY) for an integral membrane protein, followed by an open reading frame termed X which has recently been proposed to encode a new ribosomal protein (protein B). We now show that the open reading frame X indeed directs the synthesis of a protein with electrophoretic mobilities similar to the B protein, and this supports the proposal that X may be more appropriately called rpmJ. Insertion of a plasmid sequence into the secY-rpmJ boundary of the chromosome caused a reduced expression of secY probably by destabilizing the secY part of the message. The results of complementation experiments suggested that a normal level of expression of rpmJ is not required for growth or protein secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330934
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Secondary structure of the autoregulatory mRNA binding site of ribosomal protein L1 (1987)
    Springer
    Molecular genetics and genomics 210 (1987), S. 60-68 
    Details
    ISSN: 1617-4623
    Keywords: mRNA secondary structure ; Nuclease sensitivity ; Translational repressor ; Ribosomal protein ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The secondary structure of the autoregulatory mRNA binding site of Escherichia coli ribosomal protein L1 has been studies using enzymatic methods. The control region of the E. coli L11 operon was cloned into a vector under control of the Salmonella phage SP6 promoter, and RNA transcribed using SP6 RNA polymerase. The secondary structure of this RNA was probed using structure-specific nucleases, and by comparison of the data with computer predictions of RNA folding, secondary structural features were deduced. The proposed model is consistent with elements of some previously proposed models, but differs in other features. Finally, secondary structure information was obtained from two mutant mRNAs and the structural features correlated with observed phenotypes of the mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337759
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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