ISSN:
1617-4623
Keywords:
Ribosomal topography
;
Ribosomal protein L27
;
Immuno-electron microscopy
;
Peptidyl transferase centre
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Protein L27 has been localized on the ribosomal surface by immuno-electron microscopy by using antibodies specific for Escherichia coli L27, and by reconstituting 50 S subunits from an E. coli mutant, which lacks protein L27, with the homologous protein from Bacillus subtilis and using antibodies specific for the B. subtilis protein. With both approaches, protein L27 has been located at the base of the central protuberance at the interface side of the 50 S particle and thus in proximity to the peptidyl transferase centre. The immuno-electron microscopic data also suggest that the interface region of the 50 S particle is not as flat as most of the proposed three-dimensional models suggest, but instead there is a significant depression.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00327203
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