ISSN:
1432-2048
Keywords:
Chlorophyll a/b-binding protein
;
Cucumis (chloroplast protein)
;
Gene expression (light dependence)
;
Light and gene expression
;
Ribulose bisphosphate carboxylase-oxygenase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cloned complementary DNA probes have been used to measure steady-state transcript levels for the small subunit of ribulose bisphosphate carboxylase-oxygenase (SSU) and the chlorophyll a/b-binding protein (LHCP) in cotyledons during early development of cucumber seedlings. Initial accumulation of trancripts to SSU occurs 2d after germination and is independent of light and developmentally programmed. Although transcripts accumulate in dark-grown tissues, their levels increase rapidly in light-grown cotyledons from day 4, coinciding with emergence above the soil, so that by day 6 levels are 2.4 times higher in light-grown compared with dark-grown cotyledons. In contrast, accumulation of transcripts to LHCP occurs only in light-grown cotyledons. Southern blot analysis of genomic DNA indicates that in cucumber there are one and two genes encoding SSU and LHCP, respectively, considerably fewer than in those other plant species that have been examined. Both LHCP genes are expressed in light-grown cotyledons.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392386
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