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  • 1
    ISSN: 1432-2242
    Keywords: Rice ; Segregation distortion ; Wide cross ; Pollen sterility ; Wide compatibility variety
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary One or two marker genes on each of chromosomes 3, 4, 6, 7, 8, 11 and 12 of the 12 rice chromosomes were tested for segregation distortion in indica-japonica hybrids. Marker genes on chromosomes 3, 7, 8, 11 and 12 showed clear segregation distortion. This distortion was not related to the proportion of normal pollen. The germinability of the pollen was less than 10% in the hybrids, although 45–55% of the pollen grains appeared to be morphologically normal. The frequent occurrence of segregation distortion and the low germinability of the pollen grains suggested that a large portion of the pollen produced by the Indica-Japonica hybrids was not functional. The fact that the segregation distortion of the same marker may be positive or negative depending on the cross combination suggested the existence of multiple alleles, including distortion-neutral alleles. The latter mitigate pollen sterility in certain hybrid combinations.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Key words Regeneration ability ; QTL ; Rice ; Oryza sativa L. ; Seed callus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Quantitative trait loci (QTL) controlling the regeneration ability of rice seed callus were detected using 245 RFLP markers and 98 BC1F5 lines derived from two varieties, ‘Nipponbare’ and ‘Kasalath’. Regeneration ability was evaluated by two indices: average number of regenerated shoots per callus (NRS) and regeneration rate (RR). The BC1F5 lines showed continuous segregation for both indices. Five putative QTL for NRS (tentatively named qRg1, qRg2, qRg4a, qRg4b and qRg4c) located on chromosomes 1, 2 and 4 were detected. Digenic interaction among these detected QTL was not significant (P〈0.01). Among the five QTL detected, four ‘Kasalath’ alleles and one ‘Nipponbare’ allele increased NRS. According to an estimate based on the nearest marker loci, the five QTL accounted for 38.5% of the total phenotypic variation of the BC1F5 lines. For RR, four putative QTL were detected on chromosomes 2 and 4, and all of these were in the same chromosomal regions as the NRS QTL. The four RR QTL accounted for 32.6% of the total phenotypic variation.
    Type of Medium: Electronic Resource
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