ISSN:
1432-2242
Keywords:
Key words Agrobacterium tumefaciens
;
T-DNA
;
Non-T-DNA
;
Long T-DNA transfer
;
Rice transformation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The transfer of the long T-DNA (T-DNA and non-T-DNA) of a binary plasmid from Agrobacterium into the rice genome was investigated at both molecular and genetic levels. Out of 226 independent transgenic plants, 33% of the transformants contained non-T-DNA sequences. There was no major difference in the frequency of non-T-DNA transfer among three Agrobacterium tumefaciens strains.Four T1 plants containing a single putative long T-DNA insertion were selected for Southern analysis. Three of them were confirmed to have a long T-DNA insertion with a size of greater-than-unit-length of the binary plasmid. This was further confirmed by rescuing the intact binary plasmid from these plants. Our results suggest that long T-DNA transfer by rolling-circle replication from Agrobacterium to rice occurs frequently, and that the high frequency of non-T-DNA transfer should be considered when producing transgenic rice for commercial production.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050060
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