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  • 1
    Electronic Resource
    Electronic Resource
    Instability of phenotype and gene expression in long-term culture of carrot hairy root clones (1999)
    Springer
    Plant cell reports 19 (1999), S. 43-50 
    Details
    ISSN: 1432-203X
    Keywords: Key words Agrobacterium rhizogenes ; β-Glucuronidase ; Daucus carota ; gus gene ; Root culture ; NAM test ; Opines ; Gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Hairy root clones were established from carrot root discs inoculated with an agropine-type strain of Agrobacterium rhizogenes A4 harbouring the gus reporter gene on the TL-DNA. The clones were periodically examined for their phenotypic characteristics and for their ability to express the gus gene, to produce opines and to grow in the presence of NAM. The presence of the gus gene in the roots was confirmed by Southern blot hybridisation. The clones displayed various morphologies which were generally not correlated with the transformation events, and they were highly unstable throughout the successive subcultures, both for their phenotype and for their ability to express the transgenes. Reversible inactivation of the gus gene expression was associated with a high gus copy number. This could have some consequences for fundamental studies and practical uses of hairy roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050708
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  • 2
    Electronic Resource
    Electronic Resource
    In situ detection of expression of thegus reporter gene in transgenic plants: ten years of blue genes (1996)
    Springer
    Transgenic research 5 (1996), S. 281-288 
    Details
    ISSN: 1573-9368
    Keywords: reporter genes ; β-glucuronidase ; transgenic plants ; immunocytochemistry ; in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Among the methods now available to localize the sites of gene expression in plant materials, reporter genes based on thegus (uidA) gene ofEscherichia coli, which encodes a β-glucuronidase (E.C. 3.2.1.31; GUS), have been the most widely used during the last ten years. The apparent simplicity of the histochemical GUS assay has been a major factor in the increase in articles usinggus genes. However, over the last four years, there have been occasional reports expressing doubts concerning the specificity of the observed localizations based on discrepancies between results obtained with GUS histochemistry and immunocytochemistry and/orin situ hybridization. This brief review compares the results obtained with immunocytochemistry with those obtained with various GUS substrates for histochemical studies. Certain sources of artefact are discussed, as are the limits that should be imposed on interpretation of GUS histochemistry results at the organ, tissue and cell levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01968938
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    Paper (German National Licenses)
    Fulltext
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