ISSN:
1432-2048
Keywords:
Glycine (xanthine dehydrogenase)
;
Immunocytochemistry
;
Polyclonal antibody
;
Root nodule
;
Xanthine dehydrogenase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Xanthine dehydrogenase (XDH, EC 1.2.1.37) was purified from root nodules of soybean (Glycine max) and used to prepare a polyclonal rabbit antiserum. Monospecificity of this antiserum was ascertained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipate. During root nodule development of soybean, only one form of XDH was detected on an immunological basis. Titration of XDH by immunoelectrophoresis showed that a remarkable increase in the amount of XDH occurred between two and four weeks after inoculation, in parallel with the increase in enzyme activity. Localization of XDH by immunofluorescence indicated that the enzyme was present exclusively in uninfected cells where it appeared to be associated with discrete organellels
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00391414
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