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  • 1
    Electronic Resource
    Electronic Resource
    The use of semi-automated fluorescent microsatellite analysis for tomato cultivar identification (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 584-590 
    Details
    ISSN: 1432-2242
    Keywords: Key words Microsatellites ; Lycopersicon esculentum ; STMS ; Cultivar identification ; Fluorescent detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The objectives of this study were to evaluate the usefulness of a fluorescent-analysis method for genotyping PCR-based tomato microsatellite markers (or STMSs) and to establish the value of these markers to generate unique DNA profiles of tomato cultivars. The analyses were performed using forward primers labelled with a fluorochrom and using an ALF express DNA sequencer. In general, analysis of the tomato STMSs revealed distinct allelic peaks. PCR artefacts like stuttering and differential amplification were observed for several tomato STMS markers, but in most cases these artefacts did not seriously hamper allele designation. Comparison of fluorescent and silver-stained allelic profiles revealed a similar distribution of alleles among the test cultivars. Sixteen tomato cultivars were DNA-typed for 20 selected STMS markers using the fluorescent approach. Length polymorphism among the PCR products was detected with 18 of these markers, yielding gene diversity values from 0.06 to 0.74. The number of alleles per microsatellite locus ranged from 2 to 8. As few as four STMSs were sufficient to differentiate between all 16 cultivars, indicating that these markers are especially suitable for a species like tomato which has low levels of variation as detected by other types of markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050934
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Microspore culture is successful in most crop types of Brassica oleracea L. (1992)
    Springer
    Euphytica 60 (1992), S. 45-55 
    Details
    ISSN: 1573-5060
    Keywords: Brassica oleracea ; bud stage ; embryogenesis ; genetic variation ; haploidy ; microspore culture ; regeneration ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Microspore culture was shown to be applicable to a broad range of accessions belonging to six horticulturally important crop types of Brassica oleracea: broccoli, white cabbage, cauliflower, savoy cabbage, Brussels sprouts and curly kale. Of 64 accessions tested 86% were responsive. Large genotypic differences were found in number of embryos produced per flower bud, and in frequency and mode of regeneration of plants from embryos. B. oleracea was characterized by a strong asynchrony of microspore development within single buds. Microspore populations optimal for culture contained a large proportion (10–40%) of binucleate pollen. An initial high temperature treatment was essential for microspore embryogenesis. Growth conditions of the donor plants during inflorescence formation were less critical.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022257
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    Paper (German National Licenses)
    Fulltext
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