ISSN:
1573-5001
Keywords:
Triple resonance
;
Sequential assignment
;
Isotope labeling
;
Mannose permease
;
Proteins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A simplified scheme for sequential assignment in isotopically enriched proteins is presented. It is based on the standard triple resonance experiments HNCO, HN(CO)CA, HNCA and a modified H(N)CACO correlation, in which both of the HN connectivities to the CA/C′ pair of residue i and i-1 are observed. The H(N)CACO was tested on uniformly 13C/15N enriched P13 domain of mannose permease (31 kDa).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00178265
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