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Digitale Medien

Electrotransformation of intact cells ofBrevibacterium flavum MJ-233 (1990)

Satoh, Y. ; Hatakeyama, K. ; Kohama, K. ; [weitere]

Springer

Journal of industrial microbiology and biotechnology 5 (1990), S. 159-165

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Details

ISSN: 1476-5535

Schlagwort(e): Electroporation ; Shuttle vector ; Coryneform bacteria

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Summary Electroporation allowed transformation of intact cells ofBrevibacterium flavum MJ-233. The two plasmids used for electroporation were pCRY2 (6.3 kilobases) and pCRY3 (8.2 kilobases). Both plasmids contain the chloramphenicol-resistance gene and the autonomous replication origin inB. flavum MJ-233. The efficiency of electrotransformation was optimal with cells harvested at the middle log phase of growth, and was imporved by the addition of 1.0U/ml of penicillin G to the culture medium. The optimum yield of transformants per μg DNA was 5×104 when the cell suspension was pulsed at a cell density of 1×1010/ml and at a DNA amount of 1.0μg.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01573866

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