ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Rhizoferrin — a novel siderophore from the fungusRhizopus microsporus var.rhizopodiformis (1991)

Drechsel, Hartmut ; Metzger, Jörg ; Freund, Stefan ; [et al.]

Springer

BioMetals 4 (1991), S. 238-243

add to mindlist on the mindlist

Details

ISSN: 1572-8773

Keywords: Rhizoferrin ; Rhizopus ; Siderophore ; Iron transport

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Summary From a strain ofRhizopus microsporus var.rhizopodiformis a novel siderophore, named rhizoferrin, was isolated by ion-exchange column chromatography, gel filtration and preparative HPLC. Hydrolysis with 6 M HCl and subsequent gas chromatography/mass spectrometry (GUMS) of the esterified/trifluoroacetylated derivatives indicated that citric acid and diaminobutane were the only constituents. From positive fastatom-bombardment (FAB) and ion-spray tandem mass spectrometry, a molecular mass of 436 Da and the assignment of several daughter ion fragments could be obtained, which indicated the presence of two citric acid residues and one diaminobutane residue. NMR studies finally confirmedN 1,N 4-bis(1-oxo-3-hydroxy-3,4-dicarboxybutyl)-diaminobutane as the structure of rhizoferrin. The iron-binding property was demonstrated on chromeazurol S plates and its siderophore activity was confirmed by iron transport measurements in young mycelia ofR. microsporus. While rhizoferrin and also ferrioxamines B and E proved to be effective siderophores, coprogen was a poor siderophore in this fungus.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01141187

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Structure Elucidation of the Gallium-Ornibactin Complex by 2D-NMR Spectroscopy (1993)

Stephan, Holger ; Freund, Stefan ; Meyer, Jean-Marie ; [et al.]

Weinheim : Wiley-Blackwell

Liebigs Annalen 1993 (1993), S. 43-48

add to mindlist on the mindlist

Details

ISSN: 0170-2041

Keywords: Ornibactin ; Siderophore ; Iron complex ; Gallium complex ; Pseudomonas strain TVV 69 ; Chemistry ; Organic Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: From a Pseudomonas strain novel hydroxamate/carboxylate siderophores were isolated which were named ornibactins representing the modified tetrapeptide L-Orn1(Nδ-OH,Nδ-acyl)-D-threo-Asp(β-OH)-L-Ser-L-Orn4(Nδ-OH,N δ-formyl)-1,4-diaminobutane. The side-chain amino function of Orn1(Nδ-OH,Nδ-acyl) is acylated by 3-hydroxyoctanoic acid in the most lipophilic component of the microheterogeneous siderophore mixture. The structure elucidation was based on GC amino acid analysis, GC-MS and electrospray mass spectrometry of the iron complex. The 3-hydroxyoctanoic acid and the primary sequence were established on the basis of 1H and inverse detected 13C two-dimensional NMR spectra (COSY, TOCSY, NOESY, HMQC, HMBC) of the gallium-ornibactin-F complex which allowed the complete assignment of all 1H and 13C signals.

Additional Material: 10 Ill.