ISSN:
1432-136X
Keywords:
Oxygen consumption
;
Estivation
;
Metabolic depression
;
Acidosis
;
Snail,Helix aspersa
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract We have examined metabolic depression in the land snail (Helix aspersa) during estivation, and have developed a tissue model of metabolic depression using an in vitro mantle preparation. The metabolic rate ofH. aspersa is depressed by 84% in vivo within 4 weeks of onset of estivation, and this metabolic depression is accompanied by a decrease in haemolymphPO2 and pH, and an increase in haemolymphPCO2. The in vitro mantle preparation has a stable O2 consumption and energy charge, and an energy charge similar to that of mantle in vivo. The in vitro mantle is an O2-conforming tissue, withVO2 varying curvilinearly withPO2. Consequently, we have developed a mathematical method of calculating tissueVO2 at anyPO2. These calculations show that under appropriate incubation conditions of pH andPO2, the mantle from estivating animals shows a stable in vitro metabolic depression of 48% compared to mantle from control snails. The extrinsic effects ofPO2 and pH account for 70% of the total in vitro metabolic depression of mantle tissue; intrinsic effectors contribute a further 30%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02336920
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