ISSN:
1279-8509
Keywords:
Reticulocytes
;
MCV
;
Spherocytes
;
Hereditary spherocytosis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract As part of the evaluation of the GEN.S (Coulter®), we compared the Mean Corpuscular Volume (MCV) to the Mean Spherized Corpuscular Volume (MSCV) assessed during the reticulocyte count procedure under hypo-osmotic conditions. A sub-group of patients with hereditary spherocytosis (HS) was singled out in all of them, the MSCV became smaller than the MCV. As the cell volume normally increases in red cells derived from other patients in the same conditions, we decided to further study the reason for this particular behaviour of HS red cells. Whereas normal red cells are able to undergo an osmotic expansion, the spherocytes reach a critical osmotic volume leading to cell fragmentation consistent with the decrease of MSCV. This fortuitous finding is likely to be a reliable improvement for the routine screening of HS.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s00282-999-0113-8
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