ISSN:
1432-1890
Keywords:
Key words Arbuscular mycorrhiza
;
Spore nuclei
;
Fluorescence in situ hybridization
;
Ribosomal DNA loci
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Fluorescence in situ hybridization (FISH) was applied to interphasic nuclei isolated from spores of four species of AM fungi : Scutellospora castanea, Glomus mosseae, Glomus intraradices and Gigaspora rosea. Ribosomal DNA loci were visualized using digoxigenin-labeled 25 S rDNA probes obtained by nested PCR. Several hybridization sites were detected per nucleus and an internuclear variability was observed in the number of loci. This is the first report of successful application of FISH to analyse the genomes of glomalean fungi.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s005720050235
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