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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Planta 208 (1999), S. 426-430 
    ISSN: 1432-2048
    Keywords: Key words:Erysiphe (carbon source) ; Glucose transport ; Powdery mildew ; Sugar transport ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The main host carbon energy source transferred from wheat leaves (Triticum aestivum L.) to wheat powdery mildew (Erysiphe graminis f.sp. tritici) has been investigated in three ways. When the uptake of sugars by isolated mycelial suspensions was examined, the uptake rate for glucose was considerably higher than that for a range of other solutes. Analysis by high-performance liquid chromatography of leaf and mycelial extracts following uptake of sugars into infected leaf pieces confirmed that sucrose was rapidly hydrolyzed in the leaf; no sucrose or fructose could be detected in mycelial extracts. Furthermore, studies of the uptake of asymmetrically labelled sucrose indicated that this sugar is cleaved prior to uptake by the pathogen. Thus several lines of evidence show that glucose, and not sucrose, is the major carbon energy source transferred from host to fungal mycelium.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Betaine ; Lophytes ; Salt tolerance ; Suaeda
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An attempt has been made to localize glycinebetaine in shoots of Suaeda maritima L. Dum. using a technique based on the formation of an iodoplatinate precipitate. Deposits were largely restricted to the cytoplasm of salt-grown plants and were analysed by transmission analytical electron microscopy. The results are considered to support the hypothesis that glycinebetaine acts as a cytoplasmic osmoticum to balance high vacuolar salt levels in certain halophytes.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2048
    Keywords: Halophyte ; Ion accumulation ; Suaeda
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Grown under saline conditions, Suaeda maritima accumulates Na+ and Cl- into its leaves, where individual mesophyll cells behave differently in their compartmentation of these ions. Measurements of ion concentrations within selected subcellular compartments show that freeze-substitution with dry sectioning is a valuable preparative technique for analytical electron microscopy of highly vacuolate plant material. Using this approach, absolute estimates were made of Na+, K+ and Cl- concentrations in the cytoplasm, cell walls, chloroplasts and vacuoles of leaf mesophyll cells.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2048
    Keywords: Auxin ; Cell elongation ; Cytochalasin B ; Dictyosomes ; Secretion ; Triticum ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytochalasin B (CB) inhibits the elongation growth of maize roots, and that of wheat coleoptile segments incubated in indolyl-3-acetic acid, by over 30% after a lag period of about 60 min. This long lag is not due to poor tissue penetration by the inhibitor, but seems to reflect a property of the process inhibited by CB. The only visible ultrastructural change accompanying growth inhibition is the accumulation of secretory vesicles in the vicinity of dictyosomes, which occurs between 90 and 300 min. However, a massive accumulation of vesicles is seen after 120 min in root cap cells which possess very active dictyosomes. The results indicate that CB does not inhibit elongation growth by interfering with cytoplasmic streaming. Instead, they indicate that the drug acts to inhibit the secretion of cell wall components at some stage after vesicle production, but prior to their transport.
    Type of Medium: Electronic Resource
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