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  • Synaptosomes  (1)
  • Type A monoamine oxidase  (1)
  • carcinogenicity  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The effect of selective type A or type B monoamine oxidase inhibition on the intrasynaptosomal deamination of (3H)serotonin in rat spinal cord tissue (1988)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 338 (1988), S. 9-13 
    Details
    ISSN: 1432-1912
    Keywords: Serotonin metabolism ; Synaptosomes ; Type A monoamine oxidase ; Spinal cord
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The degree to which the type A and type B forms of monoamine oxidase participate in the intraneuronal deamination of (3H)serotonin (5-HT) was examined in synaptosomal-rich fractions of rat spinal cord tissue. Synaptosomes were labeled with (3H)5-HT and superfused with physiological buffers containing selective concentrations of a type A (clorgyline) or a type B (deprenyl) MAO inhibitor. The efflux of (3H)5-HT and newly-formed (3H)5-hydroxyindoleacetic acid (5-HIAA) was determined and compared to controls over time. In control samples, a slight decline in (3H)5-HT efflux occurred over the experimental superfusion period. However, a stable formation and efflux of (3H)5-HIAA was seen during this same period of time. When clorgyline was added to the superfusion buffer, a rapid decline in superfusate levels of (3H)5-HIAA was observed. Similar experiments in the presence of deprenyl were without effect. In order to elevate cytoplasmic concentrations of (3H)5-HT and therefore increase its chances for interaction with nerve terminal MAO, reserpine was added to the superfusion buffer. Reserpine caused a greater than 3-fold increase in (3H)5-HIAA formation with no change in (3H)5-HT efflux. Clorgyline inhibited this increase in (3H)5-HIAA formation but deprenyl was again without effect. In the presence of clorgyline, reserpine also caused an increase in (3H)5-HT efflux. These results strongly support the notion that 5-HT deamination within rat spinal cord nerve terminals occurs primarily, if not exclusively, through an interaction with type A MAO.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00168805
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Genotoxicity and carcinogenicity testing of 1,2-dibromopropane and 1,1,3-tribromopropane in comparison to 1,2-dibromo-3-chloropropane (1994)
    Springer
    Cell biology and toxicology 10 (1994), S. 265-279 
    Details
    ISSN: 1573-6822
    Keywords: carcinogenicity ; dibromopropane ; genetic toxicology ; mutagenicity ; tribromopropane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The activities of 1,2-dibromopropane (DBP) and 1,1,3-tribromopropane (TBP) were studied in seven genotoxicity assays, (i) SOS-induction inE. coli, (ii) DNA repair in primary rat hepatocyte culture, (iii) theSalmonella/microsome assay, (iv) a host-mediated assay usingSalmonella, (v) the somatic mutation and recombination assay inDrosophila melanogaster, (vi) HGPRT-mutagenesis assay in ARL 18 cells, and (vii) micronucleus formation assay in mouse polychromatophylic erythrocytes (PCE), forestomach (FS), glandular stomach (GS), duodenum (D), jejunum (J), cecum (C) and liver (L). The halopropanes were also tested for tumor formation in the fishDanio rerio. DBP was active in assays (ii), (v), (vii FS) and (vii L). TBP was positive in assays (ii) and (iii), strongly positive in (vii L) and borderline positive in (iv). However, neither DBP nor TBP induced tumors in fish, in contrast to the carcinogenic 1,2-dibromo-3-chloropropane. The genotoxicity and potential carcinogenicity of DBP and TBP in mammals is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00756766
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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