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  • 1
    ISSN: 1573-1561
    Keywords: Headspace ; aldehydes ; E-2-hexenal ; monoterpenes ; lipoxygenase ; spores ; hyphae ; wounding ; Lycopersicon esculentum ; Alternaria alternata ; Botrytis cinerea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A bioassay system was developed to test the effects of volatile compounds on the growth of hyphae from germinating fungal spores. Volatiles from crushed tomato leaves inhibited hyphal growth of two fungal pathogens,Alternaria alternata andBotrytis cinerea. Aldehydes, including C6 and C9 compounds formed by the lipoxygenase enzyme pathway upon wounding leaves, inhibited growth of both fungal species. Terpene hydrocarbons, 2-carene and limonene, had no significant effect on hyphal growth. The quantities of volatile compounds in the vapor phase of the bioassay system were measured by direct headspace sampling and GC analysis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1561
    Keywords: Fragaria ananassa ; two-spotted spider mite ; Tetranychus urticae ; Acari ; Tetranychidae ; headspace compounds ; methyl salicylate ; seasonal resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Headspace components from strawberry foliage have been isolated by nitrogen entrainment and Tenax trapping. Traps were eluted with hexane, and components were analyzed by gas chromatography-mass spectrometry. Fifteen compounds were identified by comparison with authentic standards,trans-2-hexenal, 1-hexanol,trans-2-hexen-1-ol,cis-3-hexen-1-ol, hexyl acetate,cis-3-hexenyl acetate, 6-methyl-5-hepten-2-ol, 1-octanol, 1-octen-3-ol, linalool, α-terpineol, methyl salicylate, ethyl saiicylate, benzyl alcohol, and 2-phenylethanol. The relative amounts of these components were compared at flowering and after fruit harvest when plants were more resistant to the two-spotted spider mite,Tetranychus urticae Koch. The predominant components,cis-3-hexen-1-ol and its acetate, did not change markedly between the sampling periods, but methyl salicylate increased approximately 10-fold after fruit harvest. Methyl salicylate at low concentrations under bioassay conditions did not affect mite behavior. The biosynthetic relationship of this compound to other phenols which have been implicated in plant resistance is discussed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-1561
    Keywords: Tetranychus urticae ; two-spotted spider mite ; Acari ; Tetranychidae ; strawberry resistance ; metabolic sink removal ; volatile foliage compounds ; headspace entrainment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An increase in resistance to the two-spotted spider mite (TSSM),Tetranychus urticae Koch, is observed in field-grown strawberry plants during the period from flowering to postharvest. This seasonal phenomenon was investigated to determine the influence of the metabolic sink, that is, fruiting in the plant. Removal of flowers and fruit and partial removal of foliage did not alter the pattern of resistance of the strawberry plant to TSSM. Bioassays were conducted in concert with chemical analyses. Headspace chemicals emitted from foliage samples were entrained in air and trapped on Tenax, identified, and compared with those entrained in nitrogen and trapped. Terpenes were among the major compounds entrained in air, whereas alcohols were obtained with nitrogen. The air-entrained headspace compounds did not appear to correlate quantitatively with the development of mite resistance in the control plants or those subjected to metabolic sink (flower and fruit) removal. Evidence was obtained for the presence of heretofore unreported strawberry foliage headspace components, namely, (Z)-3-hexenyl 2-meth-ylbutyrate, (Z)-3-hexenyl tiglate, (E)-β-ocimene, (Z)-β-ocimene, α-farnesene, and germacrene D.
    Type of Medium: Electronic Resource
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