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  • Thyroid  (1)
  • Thyroid cell  (1)
  • enterocyte  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Subcellular fractionation and subcellular localization of aminopeptidase N in the rabbit enterocytes (1986)
    Springer
    The journal of membrane biology 89 (1986), S. 53-63 
    Details
    ISSN: 1432-1424
    Keywords: aminopeptidase N ; enterocyte ; cell polarity ; fluorometry ; biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary A fast and easy procedure is proposed for preparing concomitantly from the same sample of intestinal mucosa of A+ rabbits, four fractions high enriched in the brush-border and basolateral plasma membrane domains, rough endoplasmic reticulum, and smooth endoplasmic reticulum plus Golgi apparatus membranes, respectively. This is the first time the technique of flow fluorometry has been applied to characterize the brush-border and basolateral membrane fractions using polyclonal or monoclonal antibodies against antigens common to or specific for these two plasma membrane domains. This technique definitely proves the presence of aminopeptidase in at least 60% of the basolateral membrane vesicles, where its level is about 4.5% of that in the brush-border membrane vesicles. The endoglycosidase H-sensitive intermediate of glycosylation of aminopeptidase N in the steady state is accumulated in both the rough and smooth endoplasmic reticulum membranes. Although the rough membrane is more extensive it contains only about 40% of this transient form.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01870895
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Aminopeptidase N is a marker for the apical pole of porcine thyroid epithelial cells in vivo and in culture (1981)
    Springer
    Cell & tissue research 221 (1981), S. 137-146 
    Details
    ISSN: 1432-0878
    Keywords: Aminopeptidase ; Thyroid ; Thyroid cell ; Membrane marker ; Polarization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An aminopeptidase N has been detected by immunofluorescence in the apical plasma membrane of porcine thyroid cells, facing the follicular lumen. Freshly isolated cells obtained by tissue trypsinization, lose their polarity and exhibit a homogeneous enzyme distribution over the whole plasma membrane. In thyrotropin-stimulated cultured cells organized into follicles, the enzyme is localized in the apical cell pole. In monolayer cells, on the other hand, the enzyme is distributed over the whole surface facing the medium. In both types of cultures fluorescence is also observed in intracytoplasmic organelles. In vivo, aminopeptidase is a marker of the apical part of the thyroid plasma membrane, but its in vitro localization depends upon cell differentiation related to the culture conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216576
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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