ISSN:
1040-0397
Keywords:
Tissue electrode
;
polyamine
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Oat seedling tissue and ferrocene/ferricinium were coimmobilized in a carbon paste matrix to construct a biosensor for polyamines. The oat tissue is rich in the enzymes polyamine oxidase and peroxidase while the mediator facilitates electron transfer between the electrode and hydrogen peroxide. The determination of the polyamines spermine and spermidine, in both batch and flow injection modes, was evaluated. The concentration of polyamine was monitored through hydrogen peroxide in the presence of oxygen as cosubstrate of polyamine oxidase. A reductive potential of 0.0 V was chosen for monitoring ferricinium which is generated by peroxidase. The detection limit (S/N = 3) for spermidine and spermine in the batch system was 0.19 and 1.15 μM, respectively. The relative standard deviation for twelve replicate analyses of 10 μM spermidine was 4.5%. The activity of the tissue modified carbon paste electrode decreased to 45% of the original value after a storage for 8 days in buffer solution at 4°C.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140040502
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