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  • 1
    Electronic Resource
    Electronic Resource
    Reversible inhibition of cytoplasmic streaming by intracellular Ca2+ in tonoplast-free cells ofChara australis (1981)
    Springer
    Protoplasma 109 (1981), S. 103-111 
    Details
    ISSN: 1615-6102
    Keywords: Calcium ions ; Chara ; Cytoplasmic streaming ; Internal perfusion ; Tonoplast-free cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of the intracellular concentration of Ca2+ on the cytoplasmic streaming of tonoplast-free cells ofChara australis was studied by intracellular perfusion. The perfusion media contained 1 mM Mg · ATP. Both cell ends were cut and left open. Media of different Ca2+ concentrations were perfused through the cell and the rate of the cytoplasmic streaming just after perfusion was measured. The critical concentration of Ca2+ for inhibiting the streaming was 5 × 10−4M, which was substantially higher than that found earlier byWilliamson (1975) andHayama et al. (1979). Recovery from the inhibition occurred, though not completely, by removing Ca2+. In tonoplast-free cells the Ca2+ sensitivity differed according to the culture conditions. Cells cultured indoors exhibited a higher sensitivity than those cultured outdoors. Theformer cells contained granule-rich endoplasm aggregates after loss of the tonoplast, while the latter cells did no such aggregates. The aggregates were fixed to the cortical gel with a high dosage of Ca2+ and freed by removing it.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287633
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Motive force and rate of cytoplasmic streaming inCharaceae cells in relation to osmotic pressure and ionic strength of the vacuole and the cytoplasm (1981)
    Springer
    Protoplasma 109 (1981), S. 113-125 
    Details
    ISSN: 1615-6102
    Keywords: Actin filaments ; Chara ; Cytoplasmic streaming ; Internal osmotic pressure ; Nitella ; Tonoplast-free cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of osmolarity of the vacuolar sap ofChara australis on cytoplasmic streaming was analyzed using the vacuolar perfusion technique. The osmolarity was varied between 0.3 M, which is normal and 1.2 M. The streaming rate decreased markedly with an increase in sap osmolarity, while the motive force increased significantly. This may be explained in terms of an increase in the sliding resistance at the sol-gel interface where active shearing occurs. Increase in the resistance is assumed to be caused by osmotic dehydration of the cytoplasm. This assumption was verified by the fact that in tonoplast-free cells, no significant inhibition of the streaming was observed by heightening the osmolarity of the cytoplasm with sorbitol. Heightening it with K+ salts inhibited the streaming to a greater extent than with sorbitol. The inhibition differed according to the anion species. Potassium methanesulfonate at 0.3 M and KCl at 0.6 M stopped the streaming almost completely, while 0.59 M K2SO4 was less inhibitory. Actin filaments were observed even in the presence of 0.6 M KCl.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287634
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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