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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of the Adh2 5′ region from Petunia hybrida (1996)
    Springer
    Plant molecular biology 30 (1996), S. 367-371 
    Details
    ISSN: 1573-5028
    Keywords: alcohol dehydrogenase 2 ; hypoxia ; PCR ; Petunia hybrida ; promoter ; transcript mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Adh2 gene from Petunia hybrida has been difficult to clone; exons 1 to 8 were isolated using PCR after unsuccessful screening of three genomic libraries. A combination of inverse and direct PCR strategies has been used to isolate upstream regions of Adh2. Here we report the cloning strategy for the nucleotide sequence of the 5′ region of Adh2 from P. hybrida, the locations of the transcriptional start site and putative TATA box, as well as comparative analyses of the upstream regions of petunia Adh2, other Adh genes and other genes induced by hypoxia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020123
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Insertion of Mu into the Shrunken 1 gene of maize affects transcriptional and post-transcriptional regulation of Sh1 RNA (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 135-141 
    Details
    ISSN: 1617-4623
    Keywords: Cap site ; Mutator ; Post-transcriptional regulation ; Shrunken ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Insertion of a Mu transposable element at the Shrunken 1 (Sh1) locus of maize has resulted in kernels with the typical collapsed appearance of sh mutants. Molecular analysis of the mutant gene has revealed the presence of a 1.4 kb insertion immediately upstream from the normal transcriptional start site. Mu insertion has brought about a series of changes in gene expression: the mRNA cap site has been shifted downstream so that it now lies inside the Mu element; transcription is reduced approximately sixfold. and the sh mRNA steady-state level is less than 4% of that found in the nonmutant. This disparity reflects a mutational defect in post-transcriptional regulation which is manifested as a decrease in Sh RNA abundance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00340191
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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