ISSN:
1617-4623
Keywords:
Tomato
;
Tobacco
;
Transposon tagging
;
Activator
;
Dissociation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Antibiotic resistance genes can act as either cell autonomous or non-cell autonomous genetic markers with which to monitor the excision of plant transposons. To convert spectinomycin resistance from a noncell autonomous resistance to cell autonomous resistance, a transit peptide for chloroplast localization from a petunia ribulose bisphosphate carboxylase (rbcS) gene was fused in-frame to the aadA gene, which confers spectinomycin and streptomycin resistance. Constructs were generated in which the expression of this chimeric gene was prevented by the presence, in the 5′ untranslated leader, of the maize transposons Activator (Ac) or Dissociation (Ds). When progeny of tobacco or tomato plants transformed with these constructs were germinated on spectinomycin-containing medium, germinally revertant and somatically variegated individuals could be distinguished.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00283522
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