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  • Yeast  (5)
  • Ty elements  (1)
  • 1
    Electronic Resource
    Electronic Resource
    4OS ribosomal protein from a Saccharomyces cerevisiae antisuppressor mutant exhibiting a unique 2D gel pattern (1981)
    Springer
    Current genetics 3 (1981), S. 27-29 
    Details
    ISSN: 1432-0983
    Keywords: Ribosomes ; Antisuppressor ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The yeast antisuppressor mutation, asu9-1 (Liebman and Cavenagh 1980) was found to cause an alteration in the 40S ribosomal subunit. Two-dimensional polyacrylamide gel electrophoresis patterns of the 40S ribosomal proteins from four different strains bearing the asu9-1 mutation all contained the same extra protein spot which was completely absent in five strains which did not carry the asu9 mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419577
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of novel rad6/ubc2 ubiquitin-conjugating enzyme mutants in yeast (2000)
    Springer
    Current genetics 37 (2000), S. 221-233 
    Details
    ISSN: 1432-0983
    Keywords: Key Words Rad6 ; Ubiquitination ; Silencing ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Null mutations in the RAD6/UBC2 gene encoding an E2 ubiquitin-conjugating enzyme cause deficiencies in DNA repair, N-end-rule protein degradation, sporulation and telomeric silencing, and alter the preferred integration positions for Ty1 retrotransposons. Here we selected for mutants of RAD6 that cause a release of telomeric silencing. Some alleles retained nearly wild-type ability for sporulation, DNA repair and the degradation of proteins. Alteration in Ty1 integration-site bias accompanied some of these alleles. The possibility that some mutations specifically affect binding of an unknown protein that works with Rad6 in its silencing role, but is not required for DNA repair or N-end-rule activity, is discussed in terms of the Rad6 crystal structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050523
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The DEL1 mutator gene in Saccharomyces cerevisiae does not act in trans (1985)
    Springer
    Current genetics 9 (1985), S. 259-262 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Ty1 ; Trans ; Deletion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary DEL1 strains of the yeast Saccharomyces cerevisiae exhibit a high rate of deletions of the three linked genes, CYC1, OSM1, and RAD7. Classical genetic methods showed that DELI segregated as a single Mendelian gene closely linked to CYC1. In addition, genetic evidence suggested that DEL1 was both cis- and trans-dominant (Liebman et al. 1979). Molecular analysis of deletions isolated from a haploid DEL1 strain established that deletion formation was mediated by recombination between yeast transposable elements, Ty's (Liebman et al. 1981). We now report the molecular characterization of deletions isolated from diploids in the trans configuration. This analysis reveals that these deletions probably arose in a two-step process involving mitotic recombination followed by Ty-mediated deletion formation in cis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419953
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A non-Mendelian factor, [eta+], causes lethality of yeast omnipotent-suppressor strains (1984)
    Springer
    Current genetics 8 (1984), S. 567-573 
    Details
    ISSN: 1432-0983
    Keywords: Non-Mendelian ; Yeast ; Suppressor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Omnipotent suppressors cause translational ambiguity and have been associated with poor growth and inviability. We now report that a non-Mendelian element, [eta+], causes this inviability. In [eta−] strains the suppressors are not inviable. The [eta+] genetic element segregates to about 70% of the meiotic progeny, although almost all of the spores probably have the [eta+] phenotype for the first few divisions. Growth on 5 mM guanidine hydrochloride efficiently causes [eta+] strains to become [eta−]. The [eta+] factor has many similarities with the previously described [psi+] factor (Cox 1965, 1971). However, [eta+] and [psi+] differ in their patterns of inheritance, and by the fact that [psi+] affects ochre specific and not omnipotent suppressors, while the converse is true of [eta+].
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395701
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    The same configuration of Ty elements promotes different types and frequencies of rearrangements in different yeast strains (1988)
    Springer
    Molecular genetics and genomics 211 (1988), S. 272-281 
    Details
    ISSN: 1617-4623
    Keywords: Yeast ; Ty elements ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We examined Ty-mediated genomic rearrangements in three related mitotically dividing haploid yeast strains having the same configuration of Ty elements in the CYC1-sup4 interval of chromosome X. Surprisingly, quite different types and frequencies of rearrangements were found in the three strains. In one strain we found only Ty-mediated deletions, which occurred with a frequency of about 1×10-6. Another strain yielded similar deletions, but approximately one-third of these were accompanied by adjacent Ty-mediated inversions. A third strain was found to have an extremely high rate of inversion/reinversion between two of the three Ty elements. This rate was conservatively estimated to be 1.4±0.2×10-2 per cell per generation, which is at least 2 orders of magnitude higher than previously reported values for Ty-mediated rearrangements. These data provide evidence that local regions of the genome can, in some cases, be much more fluid than had been previously believed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330604
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    Paper (German National Licenses)
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