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  • 1
    Electronic Resource
    Electronic Resource
    Chromatographic separation of asparagine-linked oligosaccharides labeled with an ultravioletabsorbing compound,p-aminobenzoic acid ethyl ester (1988)
    Springer
    Glycoconjugate journal 5 (1988), S. 13-26 
    Details
    ISSN: 1573-4986
    Keywords: asparagine-linked oligosaccharides ; UV-absorbing derivatives ; gel permeation chromatography ; HPLC ; p-aminobenzoic acid ethyl ester
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract We have expanded on the suitability ofp-aminobenzoic acid ethyl ester as an ultraviolet-absorbing reagent [Wanget al., (1984) Anal Biochem 141:366–81] for the analysis of asparagine-linked oligosaccharides derived from glycoproteins. The oligosaccharides released from glycoproteins by hydrazinolysis/N-reacetylation were derivatized withp-aminobenzoic acid ethyl ester and the derivatives were purified and separated into neutral and acidic oligosaccharides on a PRE-SEP C18 cartridge. The acidic oligosaccharides could be further separated into a few species by high-voltage paper electrophoresis. p-Aminobenzoic acid ethyl ester derivatives of neutral oligosaccharides were analyzed by gel permeation chromatography on Bio-Gel P-4 and HPLC on a silica-based amide column. The elution profile and the proportion of the oligosaccharides were in agreement with literature values. The overall yield of oligosaccharides from glycoproteins was approximately 70%. Fifty pmol of oligosaccharide were detectable on Bio-Gel P-4 and 4–5 pmol on HPLC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01048328
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  • 2
    Electronic Resource
    Electronic Resource
    Structures of asparagine linked oligosaccharides of immunoglobulins (IgY) isolated from egg-yolk of Japanese quail (1993)
    Springer
    Glycoconjugate journal 10 (1993), S. 202-213 
    Details
    ISSN: 1573-4986
    Keywords: Asn-linked oligosaccharide ; quail egg-yolk ; immunoglobulin ; p-aminobenzoic acid ethyl ester ; HPLC ; glucosylated oligomannose-type oligosaccharide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Structures of the Asn linked oligosaccharides of quail egg-yolk immunoglobulin (IgY) were determined in this study. Asn linked oligosaccharides were cleaved from IgY by hydrazinolysis and labelled withp-aminobenzoic acid ethyl ester (ABEE) afterN-acetylation. The ABEE labelled oligosaccharides were then fractionated by a combination of Concanavalin A-agarose column chromatography and anion exchange, normal phase and reversed phase HPLC before their structures were determined by sequential exoglycosidase digestion, methylation analysis, HPLC, and 500 MHz1H-NMR spectroscopy. Quail IgY contained only neutral oligosaccharides of the following categories: the glucosylated oligomannose type (0.6%, Glcα1-3Glcα1-3Man9GlcNAc2; 35.6%, Glcα1-3Man7–9GlcNAc2). oligomannose type (15.0%, with the structure Man5–9GlcNAc2) and biantennary complex type with core structures of-Manα1-3(-Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc (9.9%),-Manα1-3(GlcNAcβ1-4)(-Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc (25.1%) and-Manα1-3(GlcNAcβ1-4)(-Manα1-6)Manβ1-4GlcNAcβ1-4(Fucα1-6)GlcNAc (11.4%). Although never found in mammalian proteins, glucosylated oligosaccharides (Glc1Man7–9GlcNAc2) have been located previously in hen IgY.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00702201
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    Paper (German National Licenses)
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