ISSN:
1615-6102
Keywords:
Androgenesis
;
Embryogenesis
;
Microspore culture
;
Pollen
;
Ultrastructure
;
Wheat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have made a detailed cytological examination of the development of wheat embryoids, monitoring their initial divisions from two to ten cells by both light and electron microscopy. According to our observations the first embryogenic division is symmetrical. After the androgenesis induction treatment, there is a decrease in ribosome population with cells that have inactive nucleoli made up almost exclusively of a dense fibrillar component. This population is restored after initial embryogenic divisions. During the initial divisions the embryogenic pollen grains do not appear to change in size and the pollen wall remains intact. The exine undergoes no modification but the intine thickens, and we have observed that the thickness of the intine can be used as a cytological marker of androgenesis. The walls separating the cells obtained after embryogenic division contained numerous plasmodesmata. The beginnings of embryo polarization and cell differentiation could be made out in the very early pollen embryoids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01279902
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