ISSN:
1432-2242
Keywords:
Key words Low-molecular-weight glutenin subunits
;
A genome wheats
;
Tris-Tricine PAGE
;
Variation in genes by PCR
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A Tris-Tricine gel-electrophoresis system (Schaegger and von Jagow 1987), combined with a gradient gel, has been employed to provide an improved resolution of the B and C low-molecular-weight glutenin subunits (LMW-GSs) found in the endosperm of wheat grain. The gel system was used to document the variation in the gluten subunit proteins present in A-genome diploid wheats. The majority of LMW-GSs found in the A-genome diploid wheats were not present in normal bread wheats; the data suggest that they represent a rich source of new variation for the LMW-GSs which are considered to be very important in modulating wheat flour-processing properties. The analysis of variation in the nature of the LMW-GS genes, using PCR, demonstrated that the subclass of C-subunits assayed by primers from a previously published sequence did not show as much variation as the proteins. However, the data collected suggest that sufficient variation may exist in the LMW-GS genes of A-genome diploid wheats to use them as a source of genes for altering the flour-processing properties of hexaploid wheat.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220051048
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