ISSN:
1573-6830
Keywords:
connexin43
;
transfection
;
mRNA translation
;
in situ hybridization
;
Western blot
;
immunocytochemistry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary 1. C6 glioma cells transfected with connexin43 cDNA display a dramatic increase in the level of connexin43 mRNA and protein. 2. This overexpression of connexin43 is evident at the cellular level, as revealed within situ hybridization and immunocytochemistry. Transfection with connexin43 cDNA also induced actin stress fibers in these glioma cells. 3. Although we observed up to a 50-fold increase in the level of connexin43 mRNA following transfection, virtually all of this mRNA was present in the polysomal fraction. 4. Overexpression of connexin43 mRNA did not appear to compete with other cellular mRNAs for access to the translational machinery. 5. It is likely that the reduced proliferation rate of the transfected cells, reported earlier, is due to enhanced connexin43 expression and intercellular coupling.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00713370
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