ISSN:
1432-2242
Keywords:
Restriction fragment length polymorphism (RFLP)
;
Probe screening
;
Subspecies differentiation
;
Wide compatibility variety
;
Oryza sativa L.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Sixty-eight indica-japonica tester-differentiating RFLP probes were tested in seven indica and seven japonica varieties of rice (Oryza sativa L.) with four enzyme digestions (EcoRI, EcoRV, HindIII and DraI). Twenty-one DNA clones were isolated as indica-japonica subspecies-differentiating probes. A set of 13 probes was established as core probes for subspecies differentiation and a pooled blotting analysis was carried out to facilitate the application of RFLP in rice genetics and breeding practice. A dendrogram of 12 wide-compatibility varieties was constructed based on RFLPs detected by 13 core probes with single enzyme digestions. It was speculated that most RFLPs of indica-japonica differentiating probes were generated by insertions/deletions, which may be of great significance for the origin and differentiation of subspecies in Oryza sativa L.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00222026
Permalink
