ISSN:
1573-2568
Keywords:
acetaminophen
;
cytoprotection
;
gastric monolayer culture
;
taurocholate gastric damage
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Acetaminophen has recently been reported to protect against drug damage to gastric mucosa in vivo.The present study tested acetaminophen protection in cultured rat gastric mucous cells against sodium taurocholate-induced damage and assessed the role of endogenous prostaglandins. Cell damage was assessed by phase-contrast microscopy and quantitated by Chromium-51 release assay which positively correlated with the trypan blue dye exclusion test (r=0.98). The effect of acetaminophen on the production of PGE 2 and 6-keto-prostaglandin F 1a (6KF) was also studied. Sodium taurocholate caused cell death in a dose-dependent manner as indicated by increased 51 Cr release. Preincubation with 5 mM acetaminophen significantly reduced 51 Cr release caused by 5 mM sodium taurocholate, producing a 40% increase in cell survival. This cytoprotection was not blocked by indomethacin. PGE 2 and 6KF of the media did not change after preincubation with nondamaging concentrations of acetaminophen or taurocholate. These results indicate that: (1) acetaminophen exerts a direct protective effect on gastric mucous cells cultured in vitroindependent of indirect factors such as blood flow and (2) this protection is not associated with increased prostaglandin production.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01535988
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