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Robust refocusing of 13C magnetization in multidimensional NMR experiments by adiabatic fast passage pulses (1999)

Zweckstetter, Markus ; Holak, Tad A.

Springer

Journal of biomolecular NMR 15 (1999), S. 331-334

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ISSN: 1573-5001

Keywords: adiabatic fast passage pulses ; 13C-1H HSQC ; CBCA(CO)NH ; refocusing

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Abstract We show that adiabatic fast passage (AFP) pulses are robust refocusing elements of transverse 13C magnetization in multidimensional NMR experiments. A pair of identical AFP pulses can refocus selected parts or a complete 13 C chemical shift range in 13C spectra. In the constant time 13C-1H HSQC, replacement of attenuated rectangular pulses by selective AFP pulses results in a sensitivity enhancement of up to a factor of 1.8. In the 3D CBCA(CO)NH the signal-to-noise ratio is increased by a factor of up to 1.6.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008360013567

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