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  • 1
    Electronic Resource
    Electronic Resource
    Changes of mitochondrial membrane proteins in rat cerebellum during aging (1988)
    Springer
    Neurochemical research 13 (1988), S. 859-865 
    Details
    ISSN: 1573-6903
    Keywords: Mitochondrial membranes ; proteins ; cerebellum ; aging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Qualitative and quantitative changes of mitochondrial membrane proteind during aging were investigated. Free (non-synaptic) mitochondria were purified from rat cerebellum at different ages (4, 8, 12, 16, 20, and 24 months). Mitochondrial outer membrane (OM), inner membrane (IM) and matrix (MX) were separated and the proteins were extracted and analyzed by gel-electrophoresis. After staining, the gels were scanned densitometrically to quantify the proteins. No significant changes in the quantity of OM or MX protein subunits were observed, while serveral statistically significant quantitative changes in IM proteins with age were found. These age-dependent modifications of inner membrane mitochondrial proteins may play an important role in energy transduction, transport systems and regulatory enzymatic activities in mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00970754
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Changes of mitochondrial cytochrome c oxidase and FoF1 ATP synthase subunits in rat cerebral cortex during aging (1995)
    Springer
    Neurochemical research 20 (1995), S. 1465-1470 
    Details
    ISSN: 1573-6903
    Keywords: Cytochrome c oxidase ; FoF1 ATP synthase ; aging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The contents of subunits I, II/III, and IV of cytochrome c oxidase and of subunits α, β and γ of FoF1 ATP synthase in inner mitochondrial membrane proteins purified from cerebral cortex of rat at 2, 6, 12, 18, 24, and 26 months of age were analyzed by western blot. Age-related changes in the content of subunits, either of mitochondrial or nuclear origin, were observed. All the cytochrome c oxidase (COX) subunits examined showed an age-related increase from 2-month-old rats up to 24 months with a decrease at the oldest age (26 months). The same pattern of age-dependent changes was observed for γ ATP synthase, while the α and β subunits increased progressively up to 26 months.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00970595
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Action ofl-acetylcarnitine on age-dependent modifications of mitochondrial membrane proteins from rat cerebellum (1988)
    Springer
    Neurochemical research 13 (1988), S. 909-916 
    Details
    ISSN: 1573-6903
    Keywords: l-Acetylcarnitine ; cerebellum ; mitochondrial membranes ; proteins ; aging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Protein patterns of mitochondrial outer membrane, inner membrane, and matrix from nonsynaptic (free) mitochondria from rat cerebellum at different ages (4, 8, 12, 16, 20, and 24 months) were analyzed by gel electrophoresis. Acutel-acetylcarnitine treatment was per-formed by a single i.p. injection (100 mg/kg body weight) of the substance 60 min before the sacrifice of the animals. Different age-dependent changes were obtained for the proteins of the three fractions. The amount of some protein subunits increased and/or decreased after drug treatment. In particular, protein composition of the inner mitochondrial membrane showed significant age-related modifications. This result probably indicates differences in protein synthesis and/or turnover rates in the various mitochondrial compartments during aging. Acutel-acetylcarnitine treatment caused: a high increase in the amount of one inner membrane protein with Mw 16 kDa, at all the ages studied; a decrease in the amount of many other inner membrane proteins; modifications of some matrix proteins. Our results show that in vivo administration ofl-acetylcarnitine affects mainly the inner membrane protein composition of cerebellar mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00970761
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Changes induced by aging and drug treatment on cerebral enzymatic antioxidant system (1988)
    Springer
    Neurochemical research 13 (1988), S. 467-478 
    Details
    ISSN: 1573-6903
    Keywords: Antioxidant enzymes ; aging ; peroxidation ; drug treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The age-related modifications of the participants to the cerebral enzymatic antioxidant system (superoxide dismutase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase) were evaluated in four brain regions from male Wistar rats aged 5, 10, 15, 20, 25, 30, and 35 months. Both the specific enzyme activity and the profile of any enzyme tested markedly differ with age according to the region examined: parieto-temporal cortex, caudate-putamen, substantia nigra and thalamus. This inhomogeneous age-related profile of enzyme activities could explain both the controversial data of literature and the different regional vulnerability of the brain tissue to damage with aging. In rats aged 10, 20, or 30 months, the chronic i.p. treatment for two months with papaverine or ergot alkaloids (dihydroergocristine, dihydroergocornine, dehydroergocriptine) suggests that the antioxidant enzyme activities may be influenced according to the agent utilized, the brain region tested, and the age of the animal. In any case, small differences in the drug structure support marked differences in the type and extent of the intervention on the antioxidant enzymatic system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01268883
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Regulation of Cytochrome c Oxidase and FoF1-ATPase Subunits Expression in Rat Brain During Aging (1998)
    Springer
    Neurochemical research 23 (1998), S. 55-61 
    Details
    ISSN: 1573-6903
    Keywords: Cytochrome c oxidase ; F0F1-ATP synthase ; mRNA ; aging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In the present study we analyzed the age-dependent changes of mRNA levels for cytochrome c oxidase and F0F1-ATP synthase subunits in rat cerebral cortex and cerebellum. To establish whether the regulation of expression is transcriptional or post-transcriptional, the results were compared to those related to protein subunits levels, of the same enzymatic complexes, previously observed. The different patterns of age-related changes of mRNA subunits, in particular the lower increments, compared with those related to protein subunits, indicate that post-transcriptional mechanisms of regulation might be involved in the coordinated expression of the various subunits of each complex. Northern blotting analyses of RNA from the cerebellum of rats at the various ages, showed also differences in age-dependent patterns of transcription between cerebral cortex and cerebellum. Moreover, the major age-dependent changes of mitochondrial-encoded subunits, compared with the nuclear-encoded ones, previously observed at proteins level, occur also during transcription.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022449403619
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    Paper (German National Licenses)
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