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  • 1
    ISSN: 1615-6110
    Keywords: Leguminosae ; Medicago ; alfalfa ; Microgametophyte ; biparental inheritance ; DAPI
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microgametophytic plastid nucleoids were quantified for 18 species representing the four core genera of the tribeTrifolieae (Fabaceae),Medicago, Melilotus, Trigonella, andTrifolium. Generative cells of all taxa contained nucleoids, establishing that biparental plastid inheritance is common in theTrifolieae. Nucleoid number and volumes of pollen grains and generative cell nuclei differed among taxa. Nucleoid number was positively correlated with pollen grain and generative cell nuclear volumes, flower size and style length. These relationships disappeared after adjusting nucleoid number for pollen grain and generative cell nuclear volumes. Adjusted nucleoid numbers provided no evidence to support hypotheses that plastid content is associated with ploidy level, mating system, perenniality or size of the reproductive apparatus.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: Medicago sativa ; lucerne ; alfalfa ; callus culture ; pollen ; salt tolerance ; in vitro selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Relationships between whole-plant and germination NaCl tolerance and two in vitro measures of NaCl tolerance were evaluated in lucerne (Medicago sativa L.). No significant correlations were observed between relative NaCl tolerance of non-regenerating callus cultures, herbage regrowth, seed or pollen germination, and pollen tube growth. Simulated selection using indirect measures of NaCl tolerance produced small expected gains in relative NaCl tolerance during herbage regrowth and seed germination. These gains were less than 30% of those expected with direct selection for regrowth or germination tolerance. Selection based on the apparent NaCl tolerance of callus tissue or male gamephytes would appear unproductive. Direct selection for NaCl tolerance both at germination and during mature plant regrowth would appear to be necessary to rapidly develop lucerne populations with field-relevant levels of tolerance to NaCl stress.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 60 (1992), S. 27-35 
    ISSN: 1573-5060
    Keywords: alfalfa ; heritability ; lucerne ; Medicago sativa ; salt tolerance ; simulated selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Alfalfa (Medicago sativa L.) forage yield may be reduced by moderate levels of salinity. Increasing forage yield in saline environments through plant breeding could partially ameliorate this problem. However, prior indirect selection either in cell culture, at germination, or during seedling growth has not resulted in agronomically relevant levels of salt tolerance. This study was conducted to determine whether mean forage yield of alfalfa grown in saline environments could be increased. To reduce the number of possible selection schemes, forage yield data for five harvests from ‘Sonora’ and its parental cultivar ‘African’ grown in a greenhouse and irrigated with 0, 30, 60, or 80 mM NaCl were used to simulate one cycle of selection at each NaCl environment. Greatest gains in forage yield were expected under non-saline conditions with decreased gains under saline conditions. Selection based on yield in regrowth harvests was also predicted to be more efficient than that based on seedling harvest. Experimental populations were developed from Sonora and African at each NaCl level using phenotypic selection for increased mean forage yield for harvests 3 to 5. After each of two cycles of selection, forage yield from selected, control (plants selected randomly under non-saline conditions), and parental populations was evaluated at 0, 30, 60, and 80 mM NaCl. Gains in forage yield were observed in African when the selection and evaluation environments were the same, but in Sonora this was observed only at 0 NaCl. Selection at 0 NaCl did not increase forage yield at 60 or 80 mM NaCl in either germplasm source. This suggests that selection for increased forage yield may be successful at low to moderate NaCl levels in germplasm with ample variation for yield when grown in saline environments, while selection for increased forage yield in non-saline conditions may be ineffective.
    Type of Medium: Electronic Resource
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