ISSN:
1420-9071
Keywords:
Molecular cloning
;
fusion polypeptides
;
protein conformation
;
protein folding
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Many eukaryotic genes are cloned in bacterial hosts as fusion polypeptides. Prediction of the secondary structures for some common prokaryotic fusion polypeptides shows that many junction sites correspond to important secondary structures. It is suggested that such structures could affect (hinder, etc.) the conformation or drive the folding of the neighboring eukaryotic counterparts. Thus the prokaryotic junction should be better performed, in random coil regions, or short fusion prokaryotic polypeptides should be sued.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01951668
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