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  • animal cell  (1)
  • baculovirus expression  (1)
  • biological activity  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Biophysical and biochemical properties of baculovirus-expressed CaMV P1 protein (1992)
    Springer
    Virus genes 6 (1992), S. 5-18 
    Details
    ISSN: 1572-994X
    Keywords: CaMV movement protein ; protein characterization ; baculovirus expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cauliflower mosaic virus (CaMV) gene I encodes a protein (P1) that has been implicated in the control of virus movement in infected plants. To assist in the characterization of the mechanism of action of P1, gene I has been expressed efficiently inSpodoptera frugiperda (Sf) cells using recombinant baculovirus. Control of the expression of CaMV gene I by the polyhedrin late promotor in the baculovirusAutographa californica nuclear polyhedrosis virus (AcNPV) resulted in very high levels of P1 accumulation late in the infection cycle. This was predominantly as insoluble inclusion bodies within the cytoplasm of infected Sf cells, and not extracellularly. Evidence from anomalous gel migration and sequence homology with an analogous viral protein (tobacco mosaic virus 30K) indicated that P1 may be post-translationally processed. However, neither phosphorylation nor glycosylation of P1 occurred in this system, suggesting a functional distinction between P1 and TMV 30K. P1 from insect cells and native P1 from infected plants were immunologically related, allowing the expressed product to be used in the preparation of anti-P1 serum for detecting P1 in plant extracts. The full-size (46 kD) P1 product from insect cells, from plants, and from in vitro translations of in vitro gene I transcripts all showed similar behavior on two-dimensional protein gels, with a major pI of 7.0. Using a combination of 4 M urea, 1 M NaCl, and high temperature, P1 was solubilized. Approximately 5% of the starting material remained in solution after dialysis and remained stable to freeze/thawing. This preparation should enable us to identify the biochemical function of P1 and to resolve its role in controlling virus spread.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01703753
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  • 2
    Electronic Resource
    Electronic Resource
    Oxygen gradients in small and big sparged insect-cell bioreactors (1996)
    Springer
    Cytotechnology 20 (1996), S. 231-238 
    Details
    ISSN: 1573-0778
    Keywords: air lift ; animal cell ; bubble column ; design ; oxygen gradients ; scaleup ; stirred vessel ; CSTR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Conclusions It should be clear from the above that the calculations described here are at best rough estimations yielding order-of-magnitude values. Even though, the following general conclusions can be drawn. The gradients in stagnant layers surrounding the particles which are characteristic for animal-cell bioreactors are relatively small as compared to the gradients which can be expected in the bulk-liquid phases of the three bioreactors considered, in particular to the gradients in the stagnant layer surrounding the air bubbles. It can be concluded that under almost all circumstances gradients are likely to exist and can be very steep in larger vessels and in particular at high cell densities. The effects of gradients, however, are largely unknown; therefore research on the effects of gradients on specific and volumetric productivities and product quality seems to be an interesting area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00350403
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  • 3
    Electronic Resource
    Electronic Resource
    Selection of nuclear polyhedrosis viruses as biological control agents ofSpodoptera exigua [Lep.: Noctuidae] (1988)
    Springer
    BioControl 33 (1988), S. 299-308 
    Details
    ISSN: 1573-8248
    Keywords: Spodoptera exigua ; nuclear polyhedrosis virus ; biological control ; identification ; biological activity ; Spodoptera exigua ; Virus à polyèdres nucléaires ; lutte biologique ; identification ; activité biologique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé La virulence de 5 virus à polyèdres nucléaires à capside multiple (MNPV) contre les chenilles deSpodoptera exigua a été comparée. Trois NPV ont été isolés de chenilles mortes, trouvées dans des serres aux Pays-Bas. A partir des analyses de DNA par des enzymes de restriction il est conclu que ces 3 NPV sont apparentés aux MNPV deMamestra brassicae et sont nommés MbMNPV-NL80, MbMNPV-NL82 et MbNPV-NL83. Ces MNPVs ne sont pas apparentés au MNPV d'Autographa california (AcMNPV), ni à celui deS. exigua (SeMNPV). SeMNPV est environ 5 fois plus virulent contre les chenilles du 2e stade larvaire deS. exigua que les autres MNPV qui ont des activité biologiques comparables entre elles. Les DL-50 de SeMNPV, AcMNPV, MbMNPV-NL80, NL82 et NL83 sont 3, 18, 26, 14 et 17 polyèdres, respectivement. On en conclut que le SeMNPV est le meilleur candidat pour l'utilisation dans un programme de lutte biologique contreS. exigua dans des serres aux Pays-Bas.
    Notes: Abstract The virulence of 5 nuclear polyhedrosis viruses infectious for larvae of beet armyworm,Spodoptera exigua, was studied and their potential as biological control agents of this accidentally introduced pest in Dutch greenhouse crops is discussed. Three of the virus isolates were collected from deceased beet armyworm larvae found in Dutch greenhouses. Based on restriction endonuclease patterns of their DNA they appeared to be closely related toMamestra brassicae nuclear polyhedrosis virus (MbMNPV) and therefore were named MbMNPV-NL80, MbMNPV-NL82 and MbMNPV-NL83. These isolates were not related toAutographa californica MNPV (AcMNPV) or toSpodoptera exigua MNPV (SeMNPV), both originating from the USA. Comparison of the oiological activity of these 5 isolates showed that the SeMNPV was more virulent against beet armyworm than the other isolates. There was no significant difference in virulence between MbMNPV-NL80, NL82, NL83 and AcMNPV forS. exigua. The LD-50 values of the 5 isolates for 2nd instar larvae were 3, 26, 14, 17 and 18 polyhedra, respectively. Despite compensating qualities of the other MNPVs, such as a broader host range and potential production in alternate hosts or cell-lines, SeMNPV is considered to be the most suitable candidate as biological control agent of beet armyworm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02372619
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    Paper (German National Licenses)
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