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  • 1
    ISSN: 1573-5060
    Keywords: Brassica oleracea ; bud stage ; embryogenesis ; genetic variation ; haploidy ; microspore culture ; regeneration ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Microspore culture was shown to be applicable to a broad range of accessions belonging to six horticulturally important crop types of Brassica oleracea: broccoli, white cabbage, cauliflower, savoy cabbage, Brussels sprouts and curly kale. Of 64 accessions tested 86% were responsive. Large genotypic differences were found in number of embryos produced per flower bud, and in frequency and mode of regeneration of plants from embryos. B. oleracea was characterized by a strong asynchrony of microspore development within single buds. Microspore populations optimal for culture contained a large proportion (10–40%) of binucleate pollen. An initial high temperature treatment was essential for microspore embryogenesis. Growth conditions of the donor plants during inflorescence formation were less critical.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 43 (1996), S. 343-349 
    ISSN: 1573-5109
    Keywords: duplication ; bulking ; Brassica oleracea ; Brussels sprouts ; white cabbage ; genetic resources ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary To reduce the number of accessions in the Brassica oleracea collection of the Centre for Genetic Resources The Netherlands (CGN) groups of accessions were bulked. Accessions in a group were selections from the same landrace or old variety, and were chosen, with the help of crop experts, on the basis of their history and morphology. Since this approach was received rather sceptically in the genebank community, a genetical analysis of some of the groups and their components (named varieties) was made to justify the procedures. A selection of white cabbage and Brussels sprouts, representing two crop types of B. oleracea, was made: eleven white cabbage groups comprising 43 components, and nine Brussels sprouts groups comprising 24 components. Each group and all of its components were described on the basis of electrophoresis of nine isoenzymes on 30 plants. Most components were rightly classified; 16 percent were misclassified. All these misclassifications were within similar groups. It could be concluded that pooling of the components included in this study was justified. In two cases the isozymic patterns suggested that the groups could even have been larger. Only in the case of the two groups of Brussels sprouts ‘Bedfordshire’ this was a real option. In the other case involving the white cabbage groups ‘Langedijker Bewaar Gewoon’, ‘Langedijker Bewaar Graag’ and ‘Late Herfstdeen’ this was not an option since these landraces, although having a common genetic background, clearly have a distinct identity as defined by morphology and history.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 29 (1980), S. 273-280 
    ISSN: 1573-5060
    Keywords: Cucumber ; Cucumis sativus L. ; sex expression ; silvernitrate ; silverthiosulphate ; gibberellin ; anti-ethylene action ; male flowering induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Silvernitrate (AgNO3) and silverthiosulphate (Ag(S2O3)2 3-) effectively induced male flowering in many nodes of 7 gynoecious cucumber genotypes in 3 glasshouse trials. A single spraying of the plants in the first true leaf stage with 3 mM Ag+ as Ag(S2O3)2 3- or AgNO3 (500 ppm) yielded many more staminate flowers than GA-3 (1500 ppm) and almost as many as 3 consecutive sprayings of GA-4/7 (50 ppm). Male flowering started about 3 weeks after treatment and lasted for a period of up to 4 weeks thereafter. Plants treated with silver ions did not elongate and grew normally; effective concentrations of AgNO3 proved phytotoxic only in poor growing conditions, while Ag(S2O3)2 3- never gave deleterious side-effects. Even very strongly female lines can be induced to male flowering with silver ions, thus increasing the feasibility of large scale seed production of gynoecious × gynoecious cucumber hybrids.
    Type of Medium: Electronic Resource
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