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  • 1
    Electronic Resource
    Electronic Resource
    Genetic transformation of green bean callus via Agrobacterium mediated DNA transfer (1993)
    Springer
    Plant cell reports 12 (1993), S. 74-79 
    Details
    ISSN: 1432-203X
    Keywords: Green bean ; Phaseolus vulgaris L ; Genetic transformation ; Stable integration ; chalcone synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Kanamycin resistant callus was produced from leaf disc or hypocotyl expiants of green bean (Phaseolus vulgaris L.) when cultured on a defined medium containing 50 mg/l kanamycin after 4 days of co-cultivation with Agrobacterium tumefaciens strain EHA101 containing the binary vector pKYLX71GUS. The presence of neomycin phosphotransferase II (NPT-II) in crude cellular extracts from the kanamycin resistant callus was confirmed by ELISA. The expression of the ß-glucuronidase (GUS) reporter gene was confirmed by histochemical and fluorimetric analyses. Southern blot border analysis confirmed the integration of the foreign DNA. In addition to the evidence obtained from Southern analysis, the absence of Agrobacterium in the transformed callus cultures was confirmed by microscopic observation and through test cultures. Using the above protocol, bean callus cultures were also transformed with a bean chalcone synthase promoter-GUS fusion. These cultures, when treated with the elicitor glutathione, showed higher levels of GUS expression than the unelicited callus clumps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00241938
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Enantioselective high-performance liquid chromatography assay of (+)R- and (-)S-α-lipoic acid in human plasma (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 9 (1997), S. 32-36 
    Details
    ISSN: 0899-0042
    Keywords: chiral thiol derivatisation ; diastereomeric derivative ; reversed phase HPLC ; fluorescence detection ; assay validation ; enantiomer pharmacokinetics ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A specific plasma level assay for the enantiomers of α-lipoic acid is described. It makes use of liquid-liquid extraction, chemical reduction to the dithiol enantiomers, and their precolumn chiral derivatisation with o-phthalaldehyde in the presence of D-phenylalanine. The two diastereomeric derivatives are separated by reversed-phase HPLC with fluorescence detection. The working range of the assay is between 15 ng/ml (lower limit of quantitation) and 1,000 ng/ml for either enantiomer. Chirality 9:32-36, 1997. © 1997 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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